MoodleMoot.hu (Magyar Moodle közösség és konferencia)

• Using several trypsins to initially dissolve the antibody samples into overlapping peptides.
• Optimization of fragmentation after LC-MS/MS digestion.
• Characterizing the overlapped peptides by de novo peptide sequencing.
• Automated assembly of the antibody sequence using the characterized peptides.
• Detailed data analysis using state-of-the-art bioinformatics methods to accurately determine the amino acid sequence of the digested peptide.

Intact mass validation of the final antibody sequence.